Magnetic-activated cell sorting (MACS) is a method used to isolate and purify specific cell populations from a mixture of cells. The method uses magnetic beads that are coated with antibodies that bind to specific cell surface markers on the cells of interest. The cells are first labeled with these antibodies and then passed through a column containing the magnetic beads. The cells bound to the magnetic beads are then pulled away from the mixture by a magnetic field, while the unbound cells pass through the column. This method is commonly used to isolate cells for downstream applications such as flow cytometry, PCR, and cell culture. It has also been used to purify cells for gene therapy and regenerative medicine.

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